The 5' chemistry paragraph starts with the wrong picture (the 3' chemistry is shown...again, like in the paragraph before), and also the description is wrong. In the 5' kit from 10x Genomcis the RT starts from an oligodT primer that is NON-barcoded and NOT attached to beads (i.e., in solution). Here it's the TSO that is added with the gel beads and carrying the UMI and cell barcode.
The 5' chemistry paragraph starts with the wrong picture (the 3' chemistry is shown...again, like in the paragraph before), and also the description is wrong. In the 5' kit from 10x Genomcis the RT starts from an oligodT primer that is NON-barcoded and NOT attached to beads (i.e., in solution). Here it's the TSO that is added with the gel beads and carrying the UMI and cell barcode.
Thanks for the feedback Simone. Will revise